ColoredTaq polymerase, 500 units, 2.5u/µL, 200µL, incl. buffers

ColoredTaq polymerase is a colored version of Taq polymerase. There is a special neutral dye added to the enzyme to make it possible to track the movement of samples in gel.
Taq polymerase is a thermostable enzyme with molecular weight of approx. 94kDa. The source of this enzyme is Thermus aquaticus. The enzyme has its activity maximum at 70-74 °C (although the enzyme can work at lower temperatures of about 25 °C). Enzime's half-life at 94 °C is approx. 45 min. The enzyme catalyzes DNA synthesis in 5'->3' direction in the presence of Mg²⁺ ions and at appropriate pH. It also has a 5'->3' exonuclease activity (this activity is expressed only on double-stranded DNA). The presence of the 5'->3' exonuclease activity makes the enzyme suitable for use in RealTime PCR. Taq polymerase adds extra adenines at the 3'-end of PCR products. It does not interfere with cloning. If it is necessary to obtain PCR products without extra adenines, we recommend using Pfu polymerase. The maximum length of PCR products that can be obtained using Taq polymerase is approx. 5,000 bp.

ColoredTaq Polymerase is the best way to make PCR easier 

You can clearly see when ColoredTaq is added and how it is mixed

1. Test tube with all components added except the polymerase	2. Adding ColoredTaq. It is more dense than water	3. ColoredTaq does not mix with the solution and goes to the bottom	4. After shaking ColoredTaq mixes with the tube contents and is ready to work

When loading the reaction mix on gel it also goes down due to its higher density making the procedure more simple. With ColoredTaq it can apply amplified samples immediately onto the gel. A special dye added to the enzyme is used both as a dye for loading on the gel and as a marker showing the movement of DNA in the gel (the dye moves in a 2% gel approximately like double-stranded DNA with a length of 320 bp).   Using ColoredTaq Polymerase: • No preliminary mixing with gel loading dye • Saving time The amplicons received using ColoredTaq polymerase can be purified by standard methods . The dye added to ColoredTaq polymerase does not inhibit the work of enzymes and simplifies the PCR procedure.

Buffers included (1 mL each):

x10 Taq Buffer, 25 mM MgCl₂
700 mM Tris-HCl (pH 8.8 at 25 °C), 166 mM (NH₄)₂SO₄, 25 mM MgCl₂

x10 Taq Buffer, without MgCl₂
700 mM Tris-HCl (pH 8.8 at 25 °C), 166 mM (NH₄)₂SO₄

25 mM MgCl₂