LR Plus polymerase, 200 units, 5u/µL, 40µL, incl. buffers

LR (Long Reading) Plus polymerase is a unique recombinant enzyme for efficient amplification of long DNA fragments. The enzyme has its activity maximum at 70-74°C. LR Plus polymerase catalyzes DNA synthesis in 5'→3' direction in presence of Mg2+ ions at appropriate рН. It has also 3'→5' endonuclease activity (so-called "proofreading activity"). Presence of 3'→5' endonuclease activity makes the enzyme suitable for PCR that requires high-fidelity product synthesis.

When put into optimal conditions, LR Plus makes less errors than Taq polymerase. Amplicons synthesized with LR Plus are blunt-ended (no overhanging A at the ends). It allows for cloning of obtained product without additional treatment.

LR Plus polymerase has also 5'→3' endonuclease activity (expressed on double-stranded DNA only). Maximum length of the product obtained with LR Plus in optimal conditions is about 40000 bp.

Specific feature of LR Plus polymerase is 3'→5' endonuclease activity that makes the enzyme "slow".

LR Plus is aimed towards synthesis of long products and is not suitable for high-sensitivity PCR.

Amplification products obtained with LR Plus Polymerase   1 - 18275 bp 2 - 14118 bp 3 - 16120 bp 4 - 20329 bp M - 10 kb ladder PCR protocol: 94°C - 3 min  - 1st denaturation   35 cycles: 94°C – 20 sec  55°C – 20 sec  68°C - 25 min

Buffers included (0.5 mL each):

x10 LR Buffer, 25 mM MgCl₂
700 mM Tris-HCl (pH 9.3 at 25 °C), 166 mM (NH₄)₂SO₄, 25 mM MgCl₂

x10 LR Buffer, without MgCl₂
700 mM Tris-HCl (pH 9.3 at 25 °C), 166 mM (NH₄)₂SO₄

25 mM MgCl₂