SileksMagNA-G™ Blood DNA Isolation kit, 100 μL sample volume
The kit is designed for 100 procedures for the isolation of mainly genomic DNA from fresh and frozen blood samples.
Facts and figures:
• White blood cells, or leukocytes, are the only type of human blood cells that contain the nucleus and genomic DNA.
• Normal human blood contains 4,000 to 11,000 leukocytes per μL.
• Red blood cells (erythrocytes) of vertebrates from other classes (birds, reptiles, amphibians, various fish, etc.) have a nucleus.
- Application
- Characteristics
- Composition
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The kit is designed for 100 procedures for the isolation of mainly genomic DNA from fresh and frozen blood samples.
The principle of the method used in the kit is based on the reversible binding of nucleic acids (NA) on the surface of magnetic particles. The isolated NA can be used both in PCR and for any other molecular biological applications (labeling, cloning, sequencing, etc.). The kit contains all the necessary reagents and buffers. The extraction protocol can be modified for scaling, in the case where more NAs are required. For scaling, it is necessary to proportionally change the amount of reagents used. The use of magnetic particles enables the researcher to select the optimal amounts of materials used and to make the isolation procedure simple, fast and reproducible.
- Number of procedures100
- Starting materialvenous or capillary blood
fresh or stored for up to 1 month with a preservative or frozen and stored at -70 °C - Starting volume100 μL
- DNA yieldup to 10 μg per 100 μL
- Storage+4°C
- Transportationno special conditions required
START buffer
Lysis & Binding Buffer
Sorbent (magnetic particles)
Wash 1 buffer
Wash 2 buffer
Wash 3 buffer
Final Wash Buffer
Elution buffer