Taq polymerase, 5000 units, 5u/µL, 1mL, incl. buffers

Taq polymerase is a thermostable enzyme with molecular weight of approx. 94kDa. The source of this enzyme is Thermus aquaticus. The enzyme has its activity maximum at 70-74 °C (although the enzyme can work at lower temperatures of about 25 °C). Enzime's half-life at 94 °C is approx. 45 min. The enzyme catalyzes DNA synthesis in 5'->3' direction in the presence of Mg²⁺ ions and at appropriate pH. It also has a 5'->3' exonuclease activity (this activity is expressed only on double-stranded DNA). The presence of the 5'->3' exonuclease activity makes the enzyme suitable for use in RealTime PCR. Taq polymerase adds extra adenines at the 3'-end of PCR products. It does not interfere with cloning. If it is necessary to obtain PCR products without extra adenines, we recommend using Pfu polymerase. The maximum length of PCR products that can be obtained using Taq polymerase is approx. 5,000 bp.

Taq polymerase can be used in most cases when the enzyme for PCR is needed.
Using regular Taq polymerase allows to obtain relatively long products. It is usually required in common practice to have a proper selection of primers and reaction conditions to be able to obtain a product of such a length. Primers and reaction conditions are the primary factors required to successfully obtain a long product.
Taq polymerase can be used for all kinds of PCR, labeling reactions and sequencing.

Buffers included (10 mL each):

x10 Taq Buffer, 25 mM MgCl₂
700 mM Tris-HCl (pH 8.8 at 25 °C), 166 mM (NH₄)₂SO₄, 25 mM MgCl₂

x10 Taq Buffer, without MgCl₂
700 mM Tris-HCl (pH 8.8 at 25 °C), 166 mM (NH₄)₂SO₄

25 mM MgCl₂